A Laboratory Guide to Genomic Sequencing: The Direct Sequencing of Native Uncloned DNA Contributor(s): Saluz (Author), Jost (Author) |
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ISBN: 3764319259 ISBN-13: 9783764319250 Publisher: Birkhauser
Binding Type: Paperback Published: January 1987 Click for more in this series: Biomethods |
Additional Information |
BISAC Categories: - Science | Life Sciences - Cell Biology - Non-classifiable |
Dewey: 574.873 |
LCCN: 87029964 |
Series: Biomethods |
Physical Information: 0.36" H x 7" W x 10" L (0.67 lbs) 164 pages |
Features: Bibliography, Illustrated, Index |
Descriptions, Reviews, Etc. |
Publisher Description: A Safety Considerations Genomic sequencing involves a number of hazardous steps, such as high current, high voltage, radioactive and highly toxic chemicals. It is, therefore, absolutelyessen- tial that the instructions of equipment manufacturers be followed and that particular attention is paid to the local and federal safety regulations. INTRODUCTION 9 B Introduction During the cloning of genomic DNA many of its characteristics are perma- nently lost. It was therefore necessary to develop a new technique that would give us a closer look at a gene in its normal environment. The powerful technique of genomic sequencing, first described by Church and Gilbert (1984) now makes it possible to have a precise view of a given DNA sequence in a chromosome. This method combines the chemical DNA-sequencing procedure of Maxam and Gilbert (1980) with the detection of DNA sequences by electroblotting and indirect end-labeling by hybridization. Besides studies on the methylation state of single bases in a given gene (Nick et al., 1986; Saluz and Jost, 1986; Saluz et al., 1986), genomic sequencing can also be used to study specific DNA-protein interactions in vivo (Church et al., 1985; Giniger et al., 1985; Becker et al., 1986; Ephrussi et al., 1985; Martin et al., 1986; Nick et al., 1986; Zinn and Maniatis, 1986). |
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