Power Search
Low Price Guarantee
We Take School POs
2-D Proteome Analysis Protocols 1999 Edition
Contributor(s): Link, Andrew J. (Editor)

View larger image

 ISBN: 0896035247     ISBN-13: 9780896035249
Publisher: Humana
OUR PRICE: $161.49  

Binding Type: Hardcover - See All Available Formats & Editions
Published: September 1998
Qty:

Annotation: A step-by-step tour through the complete process of doing proteomics. With easy-to-follow instructions, complete with many helpful hints and explanations, leading investigators and pioneers in the field show how to make protein extracts, reproducibly run them on 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flatbed or vertical gels and for protein detection using autoradiography, and Coomassie, silver, and reversible metal-chelate stains. 2-D Proteome Analysis Protocols is the most complete guide for using proteomics to answer biological questions.


Click for more in this series: Methods in Molecular Biology
Additional Information
BISAC Categories:
- Science | Life Sciences - Molecular Biology
- Medical | Biochemistry
- Medical | Laboratory Medicine
Dewey: 572.6
LCCN: 98038677
Series: Methods in Molecular Biology
Physical Information: 1.5" H x 6" W x 9" L (2.38 lbs) 601 pages
Features: Bibliography, Illustrated, Index, Table of Contents
 
Descriptions, Reviews, Etc.
Publisher Description:
With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics--the study of the expressed part of the genome--has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry--especially in the fields of molecular biology, biochemistry, microbiology, and cell biology--should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.
 
Customer ReviewsSubmit your own review
 
To tell a friend about this book, you must Sign In First!